El esmalte dental bovino como modelo experimental para la investigación en odontología: una revisión de la literatura / Bovine dental enamel as experimental model for research in dentistry. A literature review

El propósito de esta revisión bibliográfica es aportar información actualizada acerca de las características de los dientes bovinos en relación con su uso como sustitutos de dientes humanos en trabajos de investigación. De acuerdo con la información registrada, los dientes bovinos serían excelentes sustitutos de la dentición humana para la realización de ensayos de laboratorio con el esmalte dental como modelo experimental (AU)

The purpose of this bibliographic review is to provide updated information about the characteristics of bovine teeth to be used as substitutes for human teeth in dental research. According to the information recorded, bovine teeth appear to be excellent substitutes for human dentition for conducting laboratory tests, using dental enamel as an experimental model (AU)

Assessment of remineralization potential of Theobromine and Sodium Fluoride gels on Artificial Caries like lesions / Avaliação do potencial de remineralização de géis de teobromina e fluoreto de sódio em lesões de cárie artificial

Objective: To compare the remineralization potential of theobromine and sodium fluoride gels on artificial caries like lesion. Materials and Methods: Forty longitudinal halves of human mandibular premolars were equally divided into 4 groups of 10 samples each: control group (C), samples were stored in distilled water during the experiment period. The remaining 30 specimens were subjected to demineralization protocol to create caries like lesions. samples were immersed for three days in a demineralization solution (pH 5.0) containing 0.2% carbopol and 0.1% lactic acid saturated with calcium phosphate tribasic. The samples were subdivided into 3 equal groups according to the treatment applied during the pH cycle. Demineralization group "D": no treatment applied. Group "F" treated with 2000 mg/liter sodium fluoride gel. Group "T" treated with 200 mg/liter theobromine gel. The specimens of the two studies groups were subjected to Demineralization- Remineralization PH Cycle Protocol for 5 days (Alternating four steps: 1: Treatment material, fluoride or theobromine ˜= 3 minutes. 2: Demineralizing solution 3 hours. 3: treatment material ˜= 3 minutes. 4: Remineralizing solution till the next cycle). The samples were investigated by scanning electron microscope (SEM) and energy dispersive x-ray analysis (EDXA). Results: The enamel of the demineralization group was porous with artificial caries like changes exposing the subsurface enamel rods with severe rod core defects. Theobromine gel and fluoride gel groups showed marked improvement in the surface characteristics in the enamel in both groups. Theobromine gel group showed more observable enamel surface improvement than the fluoride gel group. EDXA revealed that the calcium-phosphorus ratio displayed a descending order: (C > T > F > D). The differences between the two tested groups were not statistically significant. Conclusion: Theobromine gel had more effective remineralizing potential than fluoride gel as a result of its effect in improving the enamel surface characteristics of human teeth. (AU)

Objetivo: Comparar o potencial de remineralização dos géis de teobromina e fluoreto de sódio em lesões de cáries artificiais. Materiais e Métodos: Quarenta metades longitudinais de pré-molares inferiores humanos foram igualmente divididas em 4 grupos de 10 amostras cada: grupo controle (C), as amostras foram armazenadas em água destilada durante o período do experimento. As 30 amostras restantes foram submetidas ao protocolo de desmineralização paracriar lesões artificiais de cárie. As amostras foram imersas por três dias em uma solução de desmineralização (pH 5,0) contendo 0,2% de carbopol e 0,1% de ácido lático saturado com fosfato de cálcio tribásico. As amostras foram subdivididas em 3 grupos iguais, de acordo com o tratamento aplicado durante o ciclo do pH. Grupo de desmineralização "D": nenhum tratamento aplicado. Grupo "F" tratado com 2000 mg / litro de fluoreto de sódio em gel. Grupo & quot;T &q uot; tratado com 200 mg / litro de gel de teobromina. As amostras dos dois grupos de estudo foram submetidas ao protocolo de ciclo de desmineralização - remineralização por 5 dias (quatro etapas alternativas: 1: material de tratamento, flúor ou teobromina ˜= 3 minutos. 2: solução desmineralizante 3 horas. 3: material de tratamento ˜= 3 minutos 4: Solução de remineralização até o próximo ciclo). As amostras foram investigadas por microscopia eletrônica de varredura(MEV) e análise de raios-x dispersivos de energia (EDXA). Resultados: O esmalte do grupo de desmineralização era poroso, com cáries artificiais, como alterações que expunham as hastes de esmalte do subsolo com graves defeitos no núcleo da haste. Os grupos gel de teobromina e flúor apresentaram melhora acentuada nas características da superfície do esmalte nos dois grupos. O grupo gel de teobromina mostrou uma melhoria na superfície do esmalte mais observável do que o grupo gel de fluoreto. A EDXA revelou que a razão cálcio-fósforo exibia uma ordem decrescente: (C> T> F> D). As diferenças entre os dois grupos testados não foram estatisticamente significantes. Conclusão: O gel de teobromina teve um potencial remineralizante mais eficaz que o gel de flúor como resultado de seu efeito na melhoria das características da superfície do esmalte dos dentes humanos. (AU)

Efecto del blanqueamiento y el remineralizante sobre la microdureza y micromorfología del esmalte dental / Effect of whitening and remineralizing agent on the microhardness and micromorphology of dental enamel

Diversos estudios han demostrado mayor o menor grado de afectacióndel esmalte después del uso de los distintos tratamientos para blanqueamiento dental. Por este motivo, se han propuesto varios métodos ytratamientos remineralizantes que pudieran revertir los efectos negativosde dicho tratamiento. El objetivo del presente trabajo fue evaluar el efecto del Opalescence PF 15 por ciento (peróxido de carbamida al 15%) y delremineralizante Flor-Opal (NaF al 1.1 por ciento), ambos de la casa comercialUltradent, así como de la saliva artifi cial sobre la microdureza y la micromorfología del esmalte humano. Se utilizaron 10 incisivos centrales superiores recién extraídos provenientes de centros de salud del Puerto de Veracruz, México. Réplicas de estos dientes fueron obtenidas en lasdiferentes fases del tratamiento de blanqueamiento para ser posteriormente observadas en el microscopio electrónico de barrido (MEB). Asimismo, se realizaron mediciones de microdureza en la superficie del esmalte de los dientes seleccionados durante las diferentes fases del tratamiento de blanqueamiento. Se observó que la microdureza del esmalte se ve disminuida (aunque no significativamente) después de la aplicación del peróxido de carbamida al 15 por ciento, y vuelve a subir después de la aplicación de los remineralizantes, para posteriormente volver a descender siete días después del uso de los mismos. Los datos de microdureza coincidieron con los de micromorfología obtenidos enel MEB. Se concluye que el peróxido de carbamida al 15 opr ciento sí afecta lamicromorfología de la superficie del esmalte, además de que disminuyela microdureza del mismo. Con el uso del remineralizante Flor-Opal se onsiguieron mayores valores de microdureza y mejores características micromorfológicas comparado con la saliva artificial.

Studies have shown varying degrees of involvement of the enamel following the use of tooth-whitening treatments. Consequently, a range of methods and remineralizing treatments have been proposed to reverse their negative effects. The aim of this study was, therefore, to evaluate the effect of Opalescence PF 15% (carbamide peroxide 15%) and the remineralizing treatment Flor-Opal (1.1% NaF), both produced by Ultradent, as well as artifi cial saliva on the microhard-ness and micromorphology of human enamel. Ten recently-extracted central incisors from health centers in the port of Veracruz, Mexico, were used. Replicas of these teeth were made at the various stages of the whitening treatment in order to observe these under a scanning electron microscope (SEM). Microhardness measurements were also taken on the surface of the enamel of the teeth selected at each of the various stages of the whitening treatment. The microhardness of the enamel was found to decrease (though not signifi cantly) after the ap-plication of carbamide peroxide 15%, then increase again following the application of the remineralizing agents, only to decrease again seven days after these were applied. The microhardness data coincided with that for micromorphology obtained from the SEM. We conclude that carbamide peroxide 15% does affect the micromorphology of the enamel surface and also decreases its microhardness. Flor-Opal produced higher microhardness values and better micromorphological characteristics compared to artifi cial saliva.

Enamel surface evaluation after bracket debonding and different resin removal methods

OBJECTIVE: To assess enamel surface under scanning electron microscopy (SEM) after resin removal and enamel polishing procedures following brackets debonding, as well as compare the time required for these procedures. METHODS: A total of 180 deciduous bovine incisors were used. The enamel surface of each tooth was prepared and brackets were bonded with light cured Transbond XT composite resin. Brackets were removed in a testing machine. The samples were randomized and equally distributed into nine groups according to the resin removal and polishing technique: Group 1, 30-blade tungsten carbide bur in high speed; Group 2, 30-blade tungsten carbide bur in high speed followed by a sequence of 4 Sof-lex polishing discs (3M); Group 3, 30-blade tungsten carbide bur in high speed followed by Enhance tips (Dentsply). All groups were subdivided into (a) unpolished; (b) polished with aluminum oxide paste; and (c) polished with water slurry of fine pumice. Subsequently, the enamel surface was assessed and statistical analysis was carried out. RESULTS: There were statistically significant differences in enamel roughness and removal time among all groups. Groups 3a, 3b and 3c appeared to be the most efficient methods of removing resin with low damages to enamel. Groups 2a, 2b and 2c were the most time consuming procedures, and Group 2a caused more damages to enamel. CONCLUSION: The suggested protocol for resin removal is the 30-blade tungsten carbide bur in high speed followed by Enhance tips and polishing with aluminum oxide paste. This procedure seems to produce less damages and is less time consuming. .

OBJETIVO: avaliar a superfície do esmalte, por meio de microscopia eletrônica de varredura, após descolagem de braquetes e o polimento do esmalte, com diferentes técnicas de remoção de resina, bem como comparar o tempo necessário para a realização do procedimento. MÉTODOS: foram utilizados 180 dentes bovinos decíduos. A superfície do esmalte dos dentes foi preparada e realizada a colagem dos braquetes com resina Transbond XT. Os braquetes foram removidos em máquina de ensaio mecânico. Foram formados, aleatoriamente, nove grupos, de acordo com o tipo de remoção da resina e de polimento, sendo: Grupo 1, broca de tungstênio de 30 lâminas em alta rotação; Grupo 2, broca de tungstênio de 30 lâminas em alta rotação e sequência de quatro discos Soflex (3M); Grupo 3, broca de tungstênio de 30 lâminas em alta rotação e pontas de acabamento Enhance (Dentsply). Todos os grupos foram subdividido em (a) sem polimento, (b) polimento com pasta de óxido de alumínio e (c) polimento com pedra-pomes. As superfícies foram avaliadas e foi realizada análise estatística. RESULTADOS: houve diferença estatística entre os grupos em relação à rugosidade de superfície e ao tempo de remoção. Os grupos 3a, 3b e 3c demonstraram ser os métodos mais eficientes de remoção da resina, promovendo pouco dano à superfície do esmalte. Os grupos 2a, 2b e 2c consumiram maior tempo de procedimento, e o grupo 2a causou maior dano ao esmalte. CONCLUSÃO: sugere-se como protocolo de remoção o uso de broca de tungstênio de 30 lâminas para remoção do maior volume da resina, pontas de acabamento Enhance e polimento com a pasta de óxido de alumínio, por produzirem menor dano. .

The influence of display modalities on proximal caries detection and treatment decision / Influência dos meios de apresentação radiográfica no diagnóstico e tratamento da cárie

The aim of this study was to investigate the influence of digital radiographic display on caries detection and choice of treatment among undergraduate students. Forty images of extracted human teeth were acquired using a PSP digital system. The proximal surfaces were evaluated for the presence of proximal caries and choice of treatment by 36 undergraduate students,divided into three groups according to the semester they were taking. The images were evaluated in two forms of image display: laptop, and printed on acetate viewed on a lightbox.The accuracy of the different forms of image display on caries detection was evaluated by means of ROC curve analysis andits effect by mixed linear regression. Residue analysis was used to verify the adequacy of the treatment of choice for the chosen diagnosis. There was no significant effect either for the display modalities (p=0.058) or for the different undergraduate student groups (p=0.991). The Az was 0.539 for printed images and0.516 for laptop. The decisions based on treatment of choice were consistent with the scores achieved for caries detection. Accuracy of caries detection using a laptop was comparable to accuracy using printed images. Treatment decision was not affected by image display modality. The semester of the dentistry course that undergraduate students were taking did not significantly increase the accuracy of their proximal caries detection.

Objetivo deste estudo foi investigar a influência do meio de apresentação da imagem radiográfica digital no diagnóstico dacarie e na decisão de tratamento realizado por alunos de graduação. Foram obtidas 40 imagens digitais de dentes humanos extraídos através do sistema digital PSP. As superfícies proximais dos dentes foram avaliadas quanto à presença de cárie proximal por 36 estudantes de odontologia, distribuídos em três grupos de acordo com o nível de formação. As avaliações foram efetuadas em um laptop e em imagens impressas com o auxílio do negatoscópio. A acurácia dos meios de apresentação quanto à detecção de cárie incipiente foi avaliada pela média das áreas sob as curvas ROC e seu efeito por uma análise de regressão linear mista. Para a tomada de decisão terapêutica foi realizada uma análise de resíduos para verificar sua adequação ao diagnóstico. Não houve efeito significativo nem para a modalidade de visualização (p=0.058) e nem para os grupos de alunos (p=0.991). A média das áreas sob as curvas Roc para o filme foi de 0.539 e de 0.516 para negatoscópio. A decisão de tratamento foi condizente com o diagnóstico efetuado. A acurácia do diagnóstico da cárie proximal realizado em tela de laptop é comparável ao realizado em negatoscópio. A tomada de decisão terapêutica não foi afetada pelo meio de apresentação da imagem radiográfica digital. A progressão do aluno no curso não melhora a precisão diagnóstica da cárie proximal.

Ultrastructure of the surface of dental enamel with molar incisor hypomineralization (MIH) with and without acid etching / Ultraestructura de la superficie del esmalte dental con hipomineralización molar incisiva (mih) con y sin grabado ácido

El objetivo del presente trabajo fue analizar la ultraestructura y composición mineral de la superficie del esmalte de un molar con MIH, con y sin tratamiento de grabado ácido. Se analizaron una pieza dentaria permanente sin lesiones clínicas de MIH (controles) y una pieza con diagnóstico clínico de MIH leve y moderada, con indicación de extracción con y sin grabado acido (H3PO4 37 por ciento, 20”). Fueron procesadas para su observación con microscopio electrónico de barrido (SEM) ZEISS (Supra 40) y análisis de la composición mineral con detector EDS (Oxford Instruments). El esmalte del control mostró superficie prismática y patrón de grabado normales. El esmalte clínicamente sano en la pieza con MIH reveló una pérdida parcial del patrón prismático. La lesión leve se presentó porosa con ocasional esgrietas. La moderada presentó mayor porosidad, con grietas de mayor tamaño y presencia de gran cantidad de escamas. La composición mineral de las superficies afectadas mostró menor contenido de Ca y P y aumento de O y C. En la muestra con MIH, inclusive con aspecto normal, las desmineralizaciones no responden a un patrón de grabado, mostrando exposición de cristales con redondeamiento en los extremos de las varillas y menor desmineralización en los espacios interprismáticos. El grabado ácido incrementó la aparición de grietas y profundos poros en la estructura adamantina del esmalte con lesión. La composición mineral mostró en las lesiones moderadas una mayor disminución del contenido de Ca, P y Cl.El esmalte con MIH, inclusive en la superficie adamantina clínicamente intacta, presenta severas alteraciones ultraestructurales y cambios en la composición iónica afectando el patrónde grabado ácido, que podría interferir con los mecanismos de adhesión.

The aim of the present work was to analyze the ultrastructure and mineral composition of the surface of the enamel on amolar with MIH, with and without acid etching. A permanent tooth without clinical MIH lesions (control) and a tooth with clinical diagnosis of mild and moderate MIH, with indication for extraction, were processed with and without acid etching(H3PO437%, 20”) for observation with scanning electron microscope (SEM) ZEISS (Supra 40) and mineral composition analysis with an EDS detector (Oxford Instruments). The control enamel showed normal prismatic surface and etching pattern. The clinically health y enamel on the tooth with MIH revealed partial loss of prismatic pattern. The mild lesion wasporous with occasional cracks. The moderate lesion was moreporous, with larger cracks and many scales. The mineral composition of the affected surfaces had lower Ca and Pcontent and higher O and C. On the tooth with MIH, even on normal looking enamel, the demineralization does not correspond to an etching pattern, and exhibits exposure of crystals with rods with rounded ends and less demineralization in the inter-prismatic spaces. Acid etching increased the presence of cracks and deep pores in the adamantine structure of the enamel with lesion. In moderate lesions, the mineral composition had higher content of Ca, P and Cl. Enamel with MIH, even on clinically intact adamantine surfaces, shows severe alterations in the ultrastructure and changes in ionic composition, which affect the acid etching pattern and may interfere with adhesion.

Análisis descriptivo morfoestructural mediante microscopía electrónica de barrido del efecto sobre el esmalte de la técnica de grabado ácido convencional y una nueva técnica de grabado ácido aplicada en 2 tiempos operatorios / Descriptive analysis of the enamel microestructure after the conventional and a two-step modified acid etch technique using scanning electron microscopy

INTRODUCCIÓN: El grabado ácido total constituye una técnica fiable para asegurar la adhesión en el esmalte. Con una excelente adhesión al esmalte del borde cavo superficial de la preparación cavitaria se podría evitar la aparición de la filtración marginal, es por esto que se desarrolló una técnica que asegure crear un patrón eficaz de grabado en el esmalte, resultando en mayor cantidad de microrretenciones y por lo tanto, en una mejor adhesión micromecánica. MATERIALES Y MÉTODOS: En el presente estudio se utilizaron 5 dientes, todos terceros molares sanos recientemente extraídos, que fueron limpiados y cavitados para luego ser cortados por la mitad y separados en 2 grupos. El grupo A que recibiría la técnica de grabado ácido convencional y el grupo B que recibiría una variación de la técnica en 2 tiempos operatorios. Todas las muestras fueron llevadas al microscopio electrónico de barrido para observar si existen diferencias en la microestructura obtenida en cuanto al esmalte. RESULTADOS: Se observaron diferencias entre las superficies adamantinas de un mismo diente, al ser grabadas con los diferentes métodos, existiendo un patrón de grabado con mayor definición y porosidad en las muestras sometidas a grabado ácido en 2 tiempos operatorios. CONCLUSIÓN: Existen diferencias microestructurales entre las superficies adamantinas obtenidas tras el grabado según la técnica convencional y las obtenidas mediante el grabado en 2 tiempos operatorios

INTRODUCTION: Total acid etch is a reliable technique for ensuring adhesion to enamel, providing a durable adhesive interface between enamel surface and composite restoration, in order to prevent the development of micro-leakage. A variation of the etching technique was developed to create an effective and regular etching pattern. This could result in large amounts of retentions and higher surface porosity, leading to better micromechanical adhesion. MATERIALS AND METHODS: A study was conducted on Àve healthy, recently extracted third molars. They were cleaned and cavitated before being cut in half and separated into two groups. Group A received conventional etching technique and group B received a variation of the technique in a two-step procedure. All samples were observed by scanning electron microscopy (SEM) order to observe the differences in enamel microstructure between both groups. RESULTS: There were differences between the surfaces of the same tooth when etched using the different methods. There was a pattern with greater deÀnition and porosity in the samples subjected to two-step acid etching. CONCLUSION: There where microstructural differences on the enamel surfaces between samples etched by a conventional technique and those using a two-step acid etching

Enamel crystals of mice susceptible or resistant to dental fluorosis: an AFM study

Objective: This study aimed to assess the overall apatite crystals profile in the enamel matrix of mice susceptible (A/J strain) or resistant (129P3/J strain) to dental fluorosis through analyses by atomic force microscopy (AFM). Material and Methods: Samples from the enamel matrix in the early stages of secretion and maturation were obtained from the incisors of mice from both strains. All detectable traces of matrix protein were removed from the samples by a sequential extraction procedure. The purified crystals (n=13 per strain) were analyzed qualitatively in the AFM. Surface roughness profile (Ra) was measured. Results: The mean (±SD) Ra of the crystals of A/J strain (0.58±0.15 nm) was lower than the one found for the 129P3/J strain (0.66±0.21 nm) but the difference did not reach statistical significance (t=1.187, p=0.247). Crystals of the 129P3/J strain (70.42±6.79 nm) were found to be significantly narrower (t=4.013, p=0.0013) than the same parameter measured for the A/J strain (90.42±15.86 nm). Conclusion: enamel crystals of the 129P3/J strain are narrower, which is indicative of slower crystal growth and could interfere in the occurrence of dental fluorosis. .

Shear bond resistance and enamel surface comparison after the bonding and debonding of ceramic and metallic brackets

OBJECTIVE: To evaluate, in vitro, the shear bond strength presented by three brands of polycrystalline ceramic brackets and one brand of metallic bracket; verify the adhesive remnant index (ARI) after the tests, and analyze, through scanning electron microscopy (SEM) the enamel surface topography after debonding, detecting the release of mineral particles. METHODS: Sixty bovine lower incisors were used. Three ceramic brackets (Allure(r), InVu(r), and Clarity(r)) and one metallic bracket (Geneus(r)) were bonded with Transbond XT(r). Kruskal-Wallis's test (significance level set at 5%) was applied to the results of share bond and ARI. Mann Whitney's test was performed to compare the pairs of brackets in relation to their ARI. Brown-Forsythe's test (significance level set at 5%) was applied to the results of enamel chemical composition. Comparisons between groups were made with Games-Howell's and the Post-hoc tests. RESULTS: No statistically significant difference was observed in relation to the shear bond strength loads. Clarity(r) brackets were the most affected in relation to the surface topography and to the release of mineral particles of enamel (calcium ions). CONCLUSION: With regard to the ARI, there was a prevalence of score 4 (40.4%). As for enamel surface topography, the Geneus(r) bracket was the only one which did not show superficial tissue loss. The InVu(r) and Clarity(r) ones showed cohesive fractures in 33.3% and the Allure(r) in 50%, the latter being the one that presented most fractures during removal. .

OBJETIVO: avaliar, in vitro, a resistência ao cisalhamento apresentada por três marcas de braquetes ortodônticos cerâmicos policristalinos e uma marca de braquete metálico, verificando os índices de resina remanescentes (IRA) após os ensaios, e analisar, por meio de microscopia eletrônica de varredura, as topografias superficiais do esmalte após a descolagem, detectando o desprendimento de partículas minerais desse. MÉTODOS: foram utilizados 60 incisivos inferiores bovinos. Os braquetes cerâmicos (Allure, InVu e Clarity) e metálico (Geneus) foram colados utilizando-se o adesivo Transbond XT. Para os resultados referentes ao cisalhamento e ao IRA, foi utilizado o teste de Kruskal-Wallis, com nível de significância de 5%. A prova de Mann-Whtiney foi utilizada para comparar os diferentes tipos de braquetes aos pares, quanto ao IRA. Para a análise dos resultados referentes às composições químicas do esmalte, aplicou-se o teste de Brown-Forsythe, com significância de 5%. As comparações entre os grupos foram realizadas utilizando os testes Post Hoc e de Games-Hoewell. RESULTADOS: não foram observadas diferenças significativas quanto às cargas de cisalhamento. O Clarity foi o mais afetado em relação à topografia e ao desprendimento de partículas minerais do esmalte (íons Ca). CONCLUSÃO: em relação ao IRA, foi confirmada a prevalência do escore 4, com um percentual de 40,4%. Em relação à topografia superficial do esmalte, o Geneus foi o único que não apresentou perda de tecido . O InVu e o Clarity apresentaram fraturas coesivas de 33,3% de suas amostras, e o Allure de 50%, sendo o que mais fraturou durante a remoção. .

Comparative study on direct and indirect bracket bonding techniques regarding time length and bracket detachment

OBJECTIVE: The aim of this study was to assess the time spent for direct (DBB - direct bracket bonding) and indirect (IBB - indirect bracket bonding) bracket bonding techniques. The time length of laboratorial (IBB) and clinical steps (DBB and IBB) as well as the prevalence of loose bracket after a 24-week follow-up were evaluated. METHODS: Seventeen patients (7 men and 10 women) with a mean age of 21 years, requiring orthodontic treatment were selected for this study. A total of 304 brackets were used (151 DBB and 153 IBB). The same bracket type and bonding material were used in both groups. Data were submitted to statistical analysis by Wilcoxon non-parametric test at 5% level of significance. RESULTS: Considering the total time length, the IBB technique was more time-consuming than the DBB (p < 0.001). However, considering only the clinical phase, the IBB took less time than the DBB (p < 0.001). There was no significant difference (p = 0.910) for the time spent during laboratorial positioning of the brackets and clinical session for IBB in comparison to the clinical procedure for DBB. Additionally, no difference was found as for the prevalence of loose bracket between both groups. CONCLUSION: the IBB can be suggested as a valid clinical procedure since the clinical session was faster and the total time spent for laboratorial positioning of the brackets and clinical procedure was similar to that of DBB. In addition, both approaches resulted in similar frequency of loose bracket.

OBJETIVO: avaliar o tempo necessário para realização da colagem de braquetes pelas técnicas direta (CDB) e indireta (CIB), mensurando o tempo despendido entre a parte laboratorial (CIB) e a parte clínica (CDB e CIB), e a prevalência de descolamentos durante o período de observação de 24 semanas. MÉTODOS: dezessete pacientes (7 homens e 10 mulheres), com idade média de 21 anos, com necessidade de tratamento ortodôntico, foram selecionados para participar desse estudo. Foram utilizados um total de 304 braquetes, sendo que 151 foram colados pela técnica de CDB e 153 pela CIB, com o mesmo tipo de braquete e material de colagem. Os dados foram submetidos ao teste não paramétrico de Wilcoxon, com significância de 5%. RESULTADOS: o tempo total para realização da CIB foi maior em relação à CDB (p < 0,001). Levando em consideração apenas a fase clínica, foi observado que o tempo para CIB foi menor em relação a CDB (p < 0,001). A comparação entre o tempo despendido para o posicionamento dos braquetes em laboratório mais o tempo em clínica para CIB com o tempo em clínica para a CDB revelou que não houve diferença significativa (p = 0,910), nem tampouco quanto à prevalência de descolamento dos braquetes. CONCLUSÃO: a CIB apresenta-se como uma utilidade clínica singular, pois o tempo em clínica foi menor que o da CDB. O tempo despendido no posicionamento dos braquetes no laboratório e de execução clínica da CIB foi semelhante ao tempo gasto pela CDB, a quantidade de descolamentos foi semelhante entre as técnicas.

Properties of Composite Materials Used for Bracket Bonding

The purpose of this study was to evaluate in vitro the shear bond strength to enamel, flexural strength, flexural modulus, and contraction stress of one orthodontic composite and two flowable composites. Orthodontic brackets were bonded to 45 human maxillary premolars with the composites Transbond XT, Filtek Z-350 flow and Opallis flow and tested for shear bond strength. For measurement of flexural strength and flexural modulus, specimens were fabricated and tested under flexion. For the contraction stress test, cylindrical specimens were tested and an extensometer determined the height of the specimens. The data were subjected to one-way ANOVA and Tukey's test (α=0.05). The shear bond strength values were significantly lower (p<0.05) for the flowable composites compared with the orthodontic composite. For the flexural strength, no statistically significant difference was found among the composites (p>0.05) while the flexural modulus was significantly higher (p<0.05) for Transbond XT than for Filtek Z-350 flow and Opallis flow. The orthodontic composite presented significantly lower contraction stress values than the flowable composites (p<0.05). The light-activated orthodontic composite material presented higher flexural modulus and shear bond strength and lower contraction stress than both flowable composites.

O objetivo desse estudo foi avaliar a resistência ao cisalhamento no esmalte, resistência flexural, módulo flexural, tensão de contração de polimerização de duas resinas flow e uma resina ortodôntica. Os bráquetes ortodônticos foram colados em 45 pré-molares humanos e divididos: Transbond XT, Filtek Z-350 flow, Opallis flow e testado para resistência ao cisalhamento (n=15). Para a resistência e módulo flexural espécimes foram confeccionados e testados sob flexão. Para o teste de tensão de contração de polimerização, espécimes cilíndricos foram confeccionados e monitorados com um extensômetro (Instron). Os dados foram submetidos aos testes ANOVA a um critério e Tukey (α=0,05) para contraste de média. (n=15). A resistência de união das resinas flow foram significantemente menos que o da resina ortodôntica (p>0,05). A resistência flexural não demonstrou diferença significante entre os grupos testados (p>0,05). O módulo flexural da resina ortodôntica foi significantemente maior que o grupo das resinas flow (p<0,05). A tensão de contração de polimerização da resina ortodôntica foi significantemente menor (p<0,05). A resina ortodôntica obteve os maiores valores de resistência de união ao cisalhamento, resistência flexural e a menor tensão de contração de polimerização quando comparada as resinas flow testadas.

The effect of air abrasion on the retention of metallic brackets bonded to fluorosed enamel surface.

Background: Bonding brackets to fluorosed enamel remains a clinical challenge and bracket failure at the compromised enamel interface is common. Objective: To check the effect of air abrasion on the retention of metallic brackets bonded to fluorosed enamel surface. Materials and Methods: Sixty freshly extracted human premolar teeth having moderate to severe dental fluorosis as per Dean's criteria were collected and divided into three groups of 20 each. The groups were treated as follows: In group I, acid etching was followed by bonding with Transbond XT® ; in group II, sandblasting and acid etching was followed by bonding with Transbond XT® ; and in group III, sandblasting and acid etching was followed by bonding with Enlight LC® . An Instron™ universal testing machine was used for determining the debonding force, and from this the shear bond strength was computed. The sample with highest shear bond strength from each group was selected for the scanning electron microscopy (SEM) study. The prepared specimens were examined under a JSM-840A scanning electron microscope (JEOL Ltd, Tokyo, Japan) operated at 20 kV. Photographs were taken at progressively higher magnifications of ×50, ×100, ×500, and ×1000 to view the enamel surface and the adhesive remaining on the enamel surface after debonding. The shear bond strengths of the groups were compared using the one-way ANOVA (analysis of variance) and Tukey post hoc test. The distribution of adhesive remnant index (ARI) score was compared using the Chi-square test. Results: The mean shear bond strength in group I was 10.36 MPa, with a standard deviation of 0.225. The corresponding values in group II and group III were 11.41±0.237 MPa and 11.39±0.201 Mpa, respectively. There was a statistically significant difference between the three groups in the mean shear bond strength values. Conclusion: Sandblasting followed by acid etching provides significantly higher bond strength values compared to acid etching alone, irrespective of the bonding material employed.

Evaluation of the remineralization potential of amorphous calcium phosphate and fluoride containing pit and fissure sealants using scanning electron microscopy.

Aim: To evaluate the remineralization potential of Amorphous Calcium Phosphate (ACP) and Fluoride containing pit and Fissure Sealants using Scanning Electron Microscopy. Materials and Methods: Thirty maxillary first premolars were divided into three groups of ten each and were randomly selected for ACP containing (Aegis- Opaque White, Bosworth Co. Ltd.), Fluoride containing (Teethmate F1 Natural Clear, Kuraray Co. Ltd.), resin based (Concise- Opaque White, 3M ESPE Co. Ltd.) pit and fissure sealant applications. The Concise group served as a control. The teeth weresubjected to the pH-cycling regimen for a period of two weeks. After two weeks, the teeth were sectioned bucco-lingually into 4mm sections and were observed under Scanning Electron Microscope at 50X, 250X, 500X, 1000X and 1500X magnifications. The qualitative changes at the tooth surface and sealant interface were examined and presence of white zone at the interface was considered positive for remineralization. Results: Both ACP containing (Aegis) and Fluoride containing (Teethmate F1) group showed white zone at the tooth surface-sealant interface. The resin based group (Concise) showed regular interface between the sealant and the tooth structure, but no clear cut white zone was observed. Conclusion: Both, Aegis and Teethmate F1 have the potential to remineralize. Release of Amorphous Calcium Phosphate molecules in Aegis group and formation of Fluoroapetite in Teethmate F1 group, were probably responsible for the remineralization.

Clinical evaluation of the failure rate of metallic brackets bonded with orthodontic composites

The purpose of the present study was to evaluate in vivo the failure rate of metallic brackets bonded with two orthodontic composites. Nineteen patients with ages ranging from 10.5 to 38.7 years needing corrective orthodontic treatment were selected for study. The enamel surfaces from second premolars to second premolars were treated with Transbond Plus-Self Etching Primer (3M Unitek). Next, 380 orthodontic brackets were bonded on maxillary and mandibular teeth, as follows: 190 with Transbond XT composite (3M Unitek) (control) and 190 with Transbond Plus Color Change (3M Unitek) (experimental) in contralateral quadrants. The bonded brackets were light cured for 40 s, and initial alignment archwires were inserted. Bond failure rates were recorded over a six-month period. At the end of the evaluation, six bond failures occurred, three for each composite. Kaplan-Meyer method and log-rank test (Mantel-Cox) was used for statistical analysis, and no statistically significant difference was found between the materials (p=0.999). Both Transbond XT and Transbond Plus Color Change composites had low debonding rates over the study period.

O objetivo do presente estudo foi avaliar in vivo a taxa de falha de braquetes metálicos colados com dois compósitos ortodônticos. Dezenove pacientes com idades entre 10,5 e 38,7 anos, que necessitavam de tratamento ortodôntico corretivo, foram selecionados para estudo. As superfícies de esmalte de segundos pré-molares a segundos pré-molares foram tratadas com Transbond Plus Self-Etching Primer (3M Unitek). Em seguida, 380 bráquetes foram colados nos dentes superiores e inferiores, como segue: 190 com compósito Transbond XT (3M Unitek) (controle) e 190 com Transbond Plus Color Change (3M Unitek) (experimental), em quadrantes contralaterais. Os bráquetes colados foram fotopolimerizados por 40 s e arcos de alinhamento inicial foram inseridos. As taxas de insucesso na adesão foram registrados durante um período de seis meses. No final da avaliação, seis falhas de adesão ocorreram, três para cada composto. Para a análise estatística foram utilizados os testes de Kaplan-Meyer e log-rank (Mantel-Cox), e não houve diferença estatisticamente significante entre os materiais (p=0,999). Ambos compósitos, Transbond XT e Transbond Plus Color Change, tiveram baixas taxas de descolagem durante o período de estudo.

Marginal adaptation of indirect restorations using different resin coating protocols

This study evaluated the influence of material combinations used in the resin coating technique (RCT) on the marginal adaptation of indirect restorations with gingival margins in enamel (EM) and cement (CM). Eighty third-molars were used. Two cavities were prepared in each tooth. The cavities were distributed into 16 groups. Cavities with EM were filled with the following material combinations: G1: Single-Bond 2 (Sb2), G2: Sb2 + Bond/Scotchbond-Multipurpose (Sb2B), G3: Sb2 + Filtek-Flow Z350 (Sb2Fl), G4: Scotchbond-Multipurpose (SBMP), G5: Clearfil-S3 (CS3), G6: CS3 + Bond/Clearfil-SE Bond (CSE3B), G7: CS3 + Protect Liner F (CS3PL) and G8: Clearfil SE Bond + Protect Liner F (CSEBPL). The same combinations were applied to the cavities in CM: G9, G10, G11, G12, G13, G14, G15, G16, respectively. The fillings were performed with the Sinfony-System (3M/ESPE). After 24 h, the teeth were submitted to thermocycling (2,000 cycles, 5° to 55°C) and load-cycling (50,000 cycles, 50 N). Next, the Caries-Detector (Kuraray) was applied to the restoration margins. Images from the proximal margin were evaluated using the Image-Tool 3.0 software. The results were submitted to ANOVA and Tukey's test (α=0.05). The mean values (%) for the groups were: EM: G1=46.68, G2=15.53, G3=19.83, G4=27.53; G5=59.49, G6=25.13, G7=34.37, G8=15.20; CM: G9=38.38, G10=23.25, G11=26.97, G12=25.85, G13=37.81, G14=30.62, G15=29.17, G16=20.31. The highest percentages of marginal gap on EM or CM were found in the groups that did not use a liner. It can be concluded that the most appropriate RCT combinations were the groups that used a liner.

Este estudo avaliou a influência de diferentes combinações de materiais usados na técnica de selamento dentinário (TSD) sobre a adaptação marginal de restaurações indiretas, cujas margens gengivais localizam-se em esmalte (ME) ou cemento (MC). Oitenta terceiros molares foram selecionados e duas cavidades foram preparadas em cada dente, as quais foram distribuídas em 16 grupos. As cavidades com margem em esmalte foram forradas pelas seguintes combinações de materiais: G1: Single-Bond2 (Sb2), G2: Sb2 + Bond/Scotchbond-Multipurpose (Sb2B), G3: Sb2 + Filtek-Flow Z350 (Sb2Fl), G4: Scotchbond-Multipurpose (SBMP), G5: Clearfil-S3 (CS3), G6: CS3 + Bond/Clearfil-SE Bond (CSE3B), G7: CS3 + Protect Liner F (CS3PL), G8: Clearfil SE Bond + Protect Liner F (CSEBPL). As mesmas combinações foram aplicadas às cavidades com margens em cemento: G9, G10, G11, G12, G13, G14, G15, G16, respectivamente. As restaurações foram confeccionadas usando o sistema Sinfony (3M/ESPE). Após 24 h, os dentes restaurados foram submetidos à ciclagem térmica (2.000 ciclos - 5° a 55° C) e mecânica (50.000 ciclos, 50 N). Em seguida, Carie-Detector (Kuraray) foi aplicado sobre as margens das restaurações. As imagens obtidas da margem proximal foram avaliadas pelo software Image-Tool 3.0. Os resultados foram submetidos aos testes estatísticos ANOVA e Tukey (p<0,05). As médias (%) observadas para os grupos foram: ME: G1=46,68, G2=15,53, G3=19,83, G4=27,53; G5=59,49, G6=25,13, G7=34,37 e G8=15,20; MC: G9=38,38, G10=23,25, G11=26,97, G12=25,85, G13=37,81, G14=30,62, G15=29,17, G16=20,31. Os maiores valores de desadaptação marginal encontrados em ME e MC foram encontrados nos grupos que não utilizaram um "liner". Desta forma, pôde-se concluir que a combinação mais apropriada para a TSD é aquela que faz uso do "liner".

Effect of fluoride solutions on the shear bond strength of orthodontic brackets

The aim of this in vitro study was to evaluate the shear bond strength of brackets after pre-treatment with different fluoride solutions. This study used 48 freshly extracted sound bovine incisors that were randomly assigned to 4 experimental groups (n=12). CG: (control) without treatment; NF: 4 min application of neutral fluoride; APF: application of 1.23% acidulated phosphate fluoride (APF) for 4 min; and SFV: application of 5% sodium fluoride varnish for 6 h. For each group, after surface treatment, prophylaxis of enamel and bracket bonding with Transbond XT composite resin (3M) were performed following the manufacturer's specifications. The shear bond strength was performed with a universal testing machine 24 h after fixing the brackets. The tooth surfaces were analyzed to verify the adhesive remnant index (ARI). Data were analyzed statistically by ANOVA and Tukey's test (α=0.05). There was statistically significant difference among the groups (p<0.0001). CG and NF groups presented significantly higher bond strength than APF and SFV. There was no significant difference between CG and NF or between APF and SFV (p>0.05). The analysis of ARI scores revealed that most failures occurred at the enamel-resin interface. It may be concluded that the pre-treatment of enamel with 1.23% APF and 5% SFV prior to fixing orthodontic brackets reduces shear bond strength values.

O objetivo deste estudo foi o de avaliar in vitro a resistência adesiva de bráquetes após o pré-tratamento do esmalte com diferentes soluções fluoretadas. Foram utilizados 48 incisivos bovinos hígidos recém-extraídos que foram aleatoriamente divididos em 4 grupos experimentais (n=12). CG (controle): sem tratamento; FN: aplicação de flúor neutro por 4 min; FFA: aplicação de flúor fosfato acidulado (FFA) a 1,23% por 4 min; e VFS: aplicação de verniz de fluoreto de sódio a 5% por 6 h. Após o tratamento dos grupos, foi realizada a profilaxia do esmalte e os bráquetes foram fixados utilizando o compósito Transbond XT (3M), seguindo as especificações do fabricante. A resistência adesiva foi verificada através do teste de cisalhamento, realizado na Máquina de Ensaio Universal DL-2000 24 h após a fixação dos bráquetes. As superfícies dos dentes foram analisadas para verificação do índice de adesivo remanescente (IAR). Por meio dos testes estatísticos ANOVA e Tukey (α=5%), observou-se diferença estatisticamente significante entre os grupos estudados (p<0,0001), sendo que GC e FN, apresentaram valores superiores de resistência adesiva quando comparados aos grupos FFA e VFS, porém GC-NF e FFA-VFS não apresentaram diferenças estatisticas entre si (p>0,05). A análise dos escores do IAR revelou que grande parte das falhas ocorreram na interface esmalte-resina. Conclui-se que o tratamento do esmalte com FFA 1,23% e VFS a 5%, previamente à fixação de bráquetes ortodônticos, reduziu os valores de resistência adesiva ao cisalhamento.

In vitro study on tooth enamel lesions related to whitening dentifrice.

Background: The tooth whitening substances for extrinsic use that are available in Brazil contain hydrogen peroxide or carbamide peroxide. Several studies have attributed the appearance of lesions in the enamel morphology, including hypersensitivity, to these substances. Such lesions justify fluoride therapy and application of infrared lasers, among other procedures. However, there is no consensus among researchers regarding the relevance of the severity of lesions detected on the tooth surface. Objectives: The present study was carried out with an aim of evaluating in vitro the effects of the hydrogen peroxide, carbamide peroxide and sodium bicarbonate contained in dentifrice formulations, on human tooth enamel. Materials and Methods: After darkening process in laboratory, human premolars were brushed using dentifrice containing the two whitening substances (Rembrandt - carbamide peroxide and Mentadent - hydrogen peroxide) and the abrasive product (Colgate - sodium bicarbonate). The degree of specimen staining before and after this procedure was determined using spectrophotometry. Scanning electron microscopy (SEM) was used to obtain images, which were analyzed to show the nature of the lesions that appeared on the enamel surface. Results: The effectiveness of the whitening caused by hydrogen peroxide and carbamide peroxide and the abrasion caused by bicarbonate were confirmed, given that the treated test pieces returned to their original coloration. Based on SEM, evaluation of the enamel surfaces subjected to the test products showed that different types of morphologic lesions of varying severity appeared. Conclusions: Whitening dentifrice containing hydrogen peroxide and carbamide peroxide produced lesions on the enamel surface such that the greatest sequelae were associated with exposure to hydrogen peroxide.

Visualización de caries con tecnología fluorescente / Caries visualization with flouorescent technology

Los nuevos métodos de invasión mínima para el diagnóstico y el tratamiento de las enfermedades bucodentales acabarán conquistando las consultas odontológicas en el mundo. Cada vez son más los profesionales que apuestan por una práctica clínica basada en métodos no invasivos para dar respuesta a la creciente demanda de pacientes que buscan tratamientos que no resulten agresivos. La odontología de mínima intervención es una disciplina basada en la evidencia, que tiene que ver con los procedimientos conservadores en la cavidad oral, tanto en los tejidos duros como en los blandos, cuya meta principal es mejorar la calidad de vida a través de una salud oral duradera. La salud óptima de un diente se relaciona con la conservación de la mayor cantidad posible de tejido sano, pues cada procedimiento invasivo necesario para restaurar un diente, presagia su debilitamiento, especialmente si removemos más del tejido necesario. Uno de los objetivos de esta nueva corriente de Odontología, es detectar las lesiones de desmineralización en un estadio inicial; está demostrado que el uso de radiografías convencionales revelan las lesiones cuando ya está afectado considerablemente el complejo dentino-pulpar y los tratamientos que se realizan de manera convencional ponen en riesgo la integridad del diente. El presente artículo muestra uno de los nuevos sistemas de diagnóstico de caries en sus estadios iniciales, que es en los cuales se pueden realizar procedimientos restauradores muy conservadores. El sistema que se presenta es una tecnología de fluorescencia para detectar bacterias o lesiones desmineralizadas en color rojo y el esmalte sano en verde, además marca las áreas en colores y números de acuerdo a la profundidad de la lesión de una manera precisa y confiable.

Efficacy of CPP-ACP and CPP-ACPF on enamel remineralization - An in vitro study using scanning electron microscope and DIAGNOdent®.

Introduction: Remineralization as a treatment procedure has received a lot of attention both from clinicians as well researchers. The objective of this in vitro study was to find out the efficacy of casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) and casein phosphopeptide-amorphous calcium phosphate fluoride (CPP-ACPF) in remineralizing enamel surface on which artificial caries lesion had been created. The changes were analyzed using DIAGNOdent® (KaVo) and scanning electron microscope (SEM). Materials and Methods: Ninety maxillary premolars were selected and divided into three groups of 30 teeth each: A (artificial saliva), B (CPP-ACP), and C (CPP-ACPF). All the samples were assessed using DIAGNOdent® at the baseline and after demineralization and remineralization. Three samples were randomly selected from each group after remineralization for surface evaluation using SEM. Results: Statistical analysis showed that group B {CPP-ACP (4.1±1.8)} and group C {CPP-ACPF (4.8±1.2)} had a significantly higher amount of remineralization than group A (1.7±0.7). Conclusion: All the three groups showed a statistically significant amount of remineralization. However, because of the added benefit of fluoride (NaF 0.2%), CPP-ACPF (Tooth Mousse-Plus®) showed marginally more amount of remineralization than CPP-ACP (Tooth Mousse®).

Effects of thermocycling and light source on the bond strength of metallic brackets to bovine teeth

This study evaluated the effects of thermocycling and different light sources on the bond strength of metallic brackets to bovine tooth enamel using an adhesive resin. Bovine teeth were etched with 35% phosphoric acid gel for 20 s. After application of primer, metallic brackets were bonded to the buccal surface using Transbond XT, forming 8 groups (n=20), depending on the light source used for photoactivation (AccuCure 3000 argon laser - 20 s, Apollo 95E plasma arc - 12 s, UltraLume 5 LED - 40 s and XL2500 halogen light - 40 s) and experimental conditions without (Groups 1 to 4) or with thermocycling (Groups 5 to 8). Shear bond testing was carried out after 24 h of distilled water storage (Groups 1 to 4) or storage and thermocycling in distilled water (groups 5 to 8; 1,500 cycles - 5o/55oC). Data were subjected to two-way ANOVA and Tukey’s test (α=0.05). The Adhesive Remnant Index (ARI) was evaluated at ×8 magnification. No significant differences (p>0.05) in bond strength were found when the conditions without and with thermocycling were compared for any of the light sources. No significant differences (p>0.05) in bond strength were found among the light sources, irrespective of performing or not thermocycling. There was a predominance of ARI scores 1 in all groups. In conclusion, light sources and thermocycling had no influence on the bond strength of brackets to bovine enamel.

Este estudo avaliou o efeito da termociclagem e diferentes fontes de luz na resistência de união de bráquetes metálicos ao esmalte bovino utilizando uma resina adesiva. Dentes bovinos foram condicionados com ácido fosfórico 35% por 20 s. Após aplicação do primer, bráquetes metálicos foram colados na superfície bucal usando Transbond XT, formando 8 grupos (n=20), dependendo das fontes de luz usadas para fotoativação (AccuCure 3000 argon laser - 20 s, Apollo 95E plasma arc - 12 s, UltraLume 5 LED - 40 s e XL2500 halogen light - 40 s) nas condições experimentais sem (Grupos 1 a 4) ou com termociclagem (Grupos 5 a 8). O teste de resistência de união foi realizado após 24 h armazenados em água destilada (Grupos 1 a 4) ou armazenados e termociclados em água destilada (Grupos 5 a 8;1500 ciclos - 5o/55oC). Os dados foram submetidos à Análise de Variância de duas vias e ao teste de Tukey (p<0,05). O Índice Remanescente do Adesivo (IRA) foi avaliado em aumento de 8 vezes. Nenhuma diferença significante (p>0,05) na resistência de união foi encontrada quando as condições, sem ou com termociclagem foi comparado, para qualquer das fontes de luz. Nenhuma diferença significante (p>0,05) na resistência de união foi encontrada entre as fontes de luz, independente de realizar ou não a termociclagem. O IRA mostrou predominância de escore 1 em todos os grupos. Em conclusão, as fontes de luz e a termociclagem não influenciaram na resistência de união do bráquete ao esmalte bovino.